A simple, precise, accurate and economical UV visible spectrophotometric method has been developed for estimation of chloramphenicol drug by AUC method. The standard and sample solutions were prepared by using doubledistilled water as a solvent. Quantitative determination of the drug was performed at wavelength range 226-234 nm. The linearity was established over the concentration range of 5-25 µg/ml for chloramphenicol with correlation coefficient value of 0.9993. Precision studies showed that % relative standard deviation was within range ofacceptable limits. The mean percentage recovery was found to be 100.14%. The proposed method has been validated as per ICH guidelines.
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